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文献和实验). 2. Stacking buffer (4×): 0.5 M Tris-HCl, pH 6.8, and 0.4%(w/v) SDS. Store at RT. 3. 30% acrylamide/bis solution (37.5:1 with 2.6% C) andN , N , N , N ¢-tetramethyl-ethylenediamine (TEMED; Bio-Rad,Hercules, CA). 4. Ammonium persulfate: prepare 10% (w/v
protein signalling. Genes Cells , 3 ,659-670. 5. Bryja, V., Schulte, G., and Arenas, E. (2007) Wnt-3a utilizes a novel low dose and rapid pathway that does not require casein kinase 1-mediated phosphorylation of Dvl to activate beta-catenin. Cell Signal
motifs located prevalently in the 3′ untranslated regions of target messenger RNAs (mRNA). Interferon-alpha-2a (IFNα) induces a large set of protein coding genes mediating antiproliferative and antiviral responses. Here we use a global microarray-based
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