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2-8°C
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100
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| 规格： | 10ML | 产品价格： | ￥950.0 |
|---|---|---|---|
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文献和实验Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
laser. Check the target slide for an ablation spot 1-10 um in diameter. Sequentially remove ND filters until an ablation spot is seen. Adjust the ablation spot to the center of the image with the rail mirror. You will want to add back a ND filter
Freezing Embryonic Stem (ES) Cell Clones in 96-Well Plates
in a polystyrene cuvette box, cover with a polystyrene lid that fits, secure with tape, and freeze @ -70o C (the temperature optimally should drop approximately 1o C/minute). 10. For samples being screened by PCR, take the plate
/DMEM add: 1:1000 Insulin METHOD Preadipocyte maintenance and passage: We plate the cells in 10% CS/DMEM on treated polystyrene culture dishes from Corning (Cat#430167) and incubate them at 37C in 10% CO2. It is important to feed the preadipocytes
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