FITC anti-human CD8a

Isotype：human IgG1, κ

Description：CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusivelybCD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

 

Verified Reactivity：Human

Reported Reactivity：Chimpanzee, Common Marmoset

Antibody Type：Antibody Type Recombinan

Host Species：Mouse

Formulation：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation：The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions

Storage & Handling：The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application：FC-Quality tested

Excitation Laser：Blue Laser (488 nm)

Application Notes：Clone HIT8a recognizes the alpha chain of CD85. It does not block the binding of RPA-T8 antibody to CD8a.Additional reported applications of this antibody (for the relevant formats) include:immunohistochemical staining of bingtong-fixed frozen tissue sections5,6. This clone was tested in- house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.