- ￥3500 - 4500
- 欣润生物（NEWGAINBIO)
- 江苏无锡
- IH1009
- 2025年10月30日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名：
Immortalized human uterine smooth muscle cells
- 库存：
100万
- 供应商：
欣润生物
- 肿瘤类型：
否
- 细胞类型：
永生化
- ATCC Number：
无
- 品系：
人源
- 组织来源：
子宫
- 相关疾病：
无
- 物种来源：
人源
- 免疫类型：
不详
- 细胞形态：
梭形
- 是否是肿瘤细胞：
否
- 器官来源：
子宫
- 运输方式：
常温
- 年限：
/
- 生长状态：
贴壁生长
- 规格：
T25方瓶
产品介绍
产品名称：人子宫平滑肌细胞系、永生化人子宫平滑肌细胞
产品描述：子宫是动物的雌性生殖器官的一部分，是动物胎儿或幼体发育生长的场所。子宫肌层比较厚，由成束或成片的平滑肌组成，肌束间以结缔组织分隔。子宫平滑肌具有收缩功能，收缩受激素的调节，其收缩活动有助于精子向输卵管运送、经血排出以及胎儿娩出。人子宫平滑肌细胞系、永生化人子宫平滑肌细胞的分裂增殖还受性腺激素的影响。
产品货号：IH1009
产品类型: 可传代的永生化细胞
传代能力: 30代左右传代
产品形态: 梭形
培养基：人子宫平滑肌细胞系、永生化人子宫平滑肌细胞完全培养基
支原体检测：阴性
产品培养条件：37℃，5%CO2
发货方式：常温T25方瓶发货
货期：5-7天时间
Alpha SMA免疫荧光染色鉴定
Expression of Mel-CAM in implantation site intermediate trophoblastic cell line, IST-1, limits its migration on uterine smooth muscle cells.
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- 作者
- 内容
- 询问日期
文献和实验Chicken intestinal epithelial cells were obtained from NEWGAINBIO company. Cells were cultured on 37℃, with 5% CO2, in the Ham’s F-12 Nutrient (DMEM/12) that contained the following supplementations: fetal bovine serum (5%), in-sulin (5 µg/mL), transferrin (5 µg/mL), selenium (5 ng/mL), epidermal growth factor (5 ng/mL) and penicillin-streptomycin (100–100 U/mL) for cell culturing (full DMEM/12). Experiments were performed with chicken intestinal epithelial cells and working solutions were prepared with plain DMEM/12 without supplementation. For the investigations, cells were seeded onto 96-well, 24-well or 6-well polystyrene cell culture plates.
Primary hVICs (passage 2) were cultured to 50–60% confluence and infected with pGMLV-SV40T-puro lentivirus (NewgainBio, Wuxi, China) at a multiplicity of infection of 80 supplemented with 5 µg/mL polybrene (Sigma-Aldrich, Buchs, Switzerland).
Tissue was cultured until cells became visible around the tissue, and when the fusion reached 90% (FIGURE 1A) §ask ¦lled with the prepared culturing medium was sent to the company for further immortalisation. Cell immortalisation was done for cell stability and longer-term use. Immortalised cells were cultured with 10% FBS and 1% PS in the DMEM medium. After the cells multiplied and merged, they were routinely passed and grown ( NEWGAINBIO Inc. Wuxi, Jiangsu, China) (FIGURE 1B-C).
Mouse primary cultured renal vascular ECs and VSMCs were obtained from Newgainbio company, which were tested by Factor VIII and α-smooth muscle actin (α-SMA), the marker of ECs and VSMCs. RNeasy Mini Kit was used for RNA extraction, and the above protocols were repeated.
Porcine primary colon epithelial cells (Newgainbio company, Wuxi,China) were cultured in Dulbecco's Modified Eagle's Medium (Solarbio, Beijing, China) containing 10 % fetal bovine serum (BioInd, Kiryat shmona, Lsrael) at 37 ◦C and 5 % CO2 humidity.
技术资料