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文献和实验Isolation of Untouched Human CD4+ T Cells from Peripheral Blood Mononuclear Cells (PBMC)
实验原理 A mixture of mouse IgG antibodies against the non-CD4 T cells is added to the starting sample and allowed to bind to the cells. Depletion MyOne™ Dynabeads® are added and will bind to the antibodylabelled cells
together to calculate compensation in multicolor immunophenotyping experiments that incorporate a LIVE/DEAD® fixable dye by following the protocol outlined below: 1) Gently vortex the ArC™ Amine Reactive Beads Kit and the AbC™ Anti-Mouse Bead Kit
. 2. Combining AbC™ anti-Rat/Hamster and ArC™ Amine Reactive Compensation Bead Kits The ArC™ Amine Reactive Compensation Bead Kit (Cat. no. A10346) is designed to facilitate compensation when using any of the LIVE/DEAD® fixable dead cell stains
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