- ￥6125
- 赛默飞（Thermo Fisher）
- PA5143643
- 2025年07月15日
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文献和实验Generation and Application of Phospho-specific Antibodies for p53 and pRB
of phosphoproteins. We have improved methods for generation of phosphorylation site-specific antibodies and have successfully obtained antibodies for the analysis of most of the phosphorylation sites on p53 and RB proteins.
(see below) and pooled. 3. Dot blots to identify actin in the DE-53 column fractions are performed by spotting 2 ml of each fraction tested to a sheet of nitrocellulose marked with a grid pattern in pencil. After the samples were air dried, the nitrocellulose
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
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