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文献和实验membrane, creating a structure similar to an ion channel Delivers early signal in B cell activation, allowing resting B cells to respond to later antigens Rituximab is a chimeric murine-human anti-CD20 antibody used for treatment of B cell
environment with little denaturation. Purified antibodies: Once purified, if the antibody is at less than 0.1 mg/ml add a small amount of detergent or protein (0.1% TWEEN-20 or 1% bovine serum albumin) and some protease inhibitors to the solution
(5) 10X PBS80 g NaCl2 g KCl14.4 g Na2HPO42.4 g KH2PO4up to 1 liter with Q(6) Affigel Blue Regeneration Buffer2 M guanidine HCl or 1.5 M Na thiocyanate(7) HiTrap Storage Buffer10 mM Tris 7.5 1 ml 1M Tris 7.50.1 mM EDTA 20 ul 500 mM EDTA50 mM NaCl 1 ml 5M
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