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文献和实验Methods for the Investigation of Thyroid Function
of free thyroxine. An equilibrium dialysis method is described. Methods for the measurement of TSH receptor antibodies and for thyroid peroxidase and thyroglobulin antibodies used in the assessment of autoimmune thyroid disease are given.
Methods of Measuring Thyroglobulin and Peptide-Methylcoumarinamide Hydrolysis by Autoantibodies
% of the total protein in this gland. The protein is iodinated within the thyroid by peroxidase-catalyzed reactions and proteolytic processing of the iodinated Tg leads to formation of the thyroid hormones T3 and T4 . Autoantibodies to Tg are found in >80
免疫组化技术-Immunohistochemical Protocols
Before the incubation with the primary antibody, the endogenous peroxidase activity is blocked by a 30 min incubation in 3% H 2 O 2 in PBS (pH 7.4). The sections are washed in 3x5 min PBS
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