- ￥5847
- 赛默飞（Thermo Fisher）
- PA5141985
- 2025年06月26日
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文献和实验In Situ Immunofluorescence Analysis: Immunofluorescence Microscopy
of this method is dependent on the quality and specificity of the antibodies and the relative stability of antigens. Generally, the overall scheme for localization of cellular proteins involves fixation and permeabilization of cells for antibody accessibility
免疫组化技术-Immunohistochemical Protocols
it as a marker to search for the antigen in tissue. The antibody can be directly conjugated to a fluorochrome or biotin that can then be detected in several different ways. Much more common, however, is the use of a non-conjugated primary antibody
. PBTB solution: 1x PBS, 0.1% Tween-20, 1% milk powder. 4. Detection of DIG-labeled probe: a. Biotinylated anti-DIG antibody followed by Streptavidin-HRP, recommended to obtain strongest signal: Biotin-conjugated mouse
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