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文献和实验Rapid Isotyping of Mouse Monoclonal Antibodies
from serum, valence, and avidity, all depend on isotype. Isotyping is a basis for the selection of antibody purification, conjugation, fragmentation, and storage procedures, and predicts cytotoxic properties and half-life in vivo (see Note 1 ). In addition
of several target mRNAs from each sample. This procedure was optimized on the human erythroleukemia cell line TF-1 but was successfully used on primary cells and on different cell lines. We describe the detailed procedure for the analysis of Bcl-2 levels. Aldolase
Semiquantitative RT-PCR analysis to assess the expression levels of multiple…
-1 but was successfully used on primary cells and on different cell lines. We describe the detailed procedure for the analysis of Bcl-2 levels. Aldolase A was used as an internal control to normalize for sample to sample variations in total RNA amounts and for reaction
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