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- 赛默飞（Thermo Fisher）
- PA5109300
- 2025年07月12日
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文献和实验Viral Glycoprotein-Mediated Cell Fusion Assays Using Vaccinia Virus Vectors
The vaccinia virus-based expression of viral envelope glycoprotein genes—derived from enveloped viruses that infect their respective host cells through a pH-independent mechanism of membrane fusion—has been a powerful tool in helping
Expression, Glycosylation, and Modification of the Spike (S) Glycoprotein of SARS CoV
glycoprotein can be deduced from its sensitivity to hydrolysis by endoglycosidase H (EndoH) or N -glycosidase F (N-Gly F). A fully matured S glycoprotein will be modified with complex oligosaccharides which makes it resistant to cleavage by EndoH but not by N
Analysis of Fusion Using a Virus-Free Cell Fusion Assay
is quantitated by the amount of luciferase expression after mixing of the two cell types. Alongside the fusion assay, a CELISA is performed to determine glycoprotein expression on the effector cells. This methodology has been useful in studying membrane fusion
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