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文献和实验: 1. Prepare Whole Cell Extract. Make sure every solution DOES NOT contain DTT. 2. Add 1 or 2 μl of non-diluted monoclonal antibody (4B4 is better than 10H8 for HSF1) or 1/10 diluted polyclonal antibody to 10 μg of whole cell extract
这四个位点包括他们首次发现的两个具有全基因组显著相关水平位点:BIN1附近的rs744373(OR,1.13, 95%CI:1.06-1.21每次要等位基因拷贝,p=1.59*10^-11),EXOC3L2/BLOC1S3/MARK4附近的rs597667 (OR, 1.18; 95% CI, 1.07-1.29; P = 6.45x10–9)。虽然在独立样本中确定了CLU和PICALM与AD相关,但这两个基因的位点并没有改善一个包含年龄,性别和APOE在内的预测AD发病模型的功能
Western Analysis of Transgenic Plants
are transferred to a solid support, which is normally nitrocellulose or polyvinylidene fluoride (PVDF [Millipore, Bedford, MA]). Immunological detection of proteins transferred to a solid support combines ease of handling with accessibility of antibodies
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