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文献和实验High-Performance Liquid Chromatography Analysis of CYP2C8-Catalyzed Paclitaxel 6-Hydroxylation
. A reverse-phase, high-performance liquid chromatographic assay is described for the analysis of paclitaxel 6α-hydroxylation catalyzed by human liver microsomes or cDNA-expressed P450 enzyme CYP2C8. Analytical separations are achieved using a C18 column
High-Performance Liquid Chromatographic Analysis of CYP2CSCatalyzed Paclitaxel 6-Hydroxylation
CYP2C8 is a major CYP2C protein expressed in human liver 1 –4 . Considerable interindividual differences (-20-fold) have been observed in hepatic CYP2C8 content (5 ) and a blmodal dlstrlbutlon in CYP2C8 protein amounts m a panel of human
CYP2C 19 is one of at least three CYP2C enzymes expressed in human hver (1 , 2 ), although the abundance of this P450 is generally less than that of etther CYP2C8 or CYP2C9 (3 ). Considerable interindividual differences occur in hepatic
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