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文献和实验Immunohistochemistry: Fluorescence Protocol 2.0
by centrifugation (400 x g for 5 minutes). Incubate the cells for 30 minutes at room temperature with 50 µL of a biotinylated secondary antibody (either biotin-donkey anti-goat IgG Fab2 diluted 1:700 or biotin-goat anti-mouse IgG1 or IgG2A or IgG2B diluted
Immunohistochemisty-Fluorescence Protocol
: If using R&D Systems biotinylated antibody skip steps 9 and 10 and continue. Incubate each cell spot on slides for 30 minutes at room temperature with 15 µL of a biotinylated secondary antibody (either biotin-donkey anti-goat IgG Fab2 diluted
【资源】全面归类网址:毕赤酵母表达各种来源蛋白(细菌,真菌,植物,人类等)
hedgehog fused to murine and rat IgG1 hinge and FcS, α-MF, active508ThrombomodulinS[274]Tissue factor extracellular domainS, 10 mg/L, PHO1[275]Tissue kallikreinS, 30 mg/L, α-MF[276, 277]Tissue-type plasminogen activator kringle 2 domainS, 170 mg/L, α-MF[278
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