- ￥4296
- 赛默飞（Thermo Fisher）
- PA146294
- 2025年07月16日
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文献和实验Pulse-Chase mRNA decay assay in HeLa Tet-off cells:
This protocol is for four time-points in 12-well plates. Scale up or down as needed. Day 1: Plate cells: Split one or more 10-cm plate(s) with 80-100% confluent HeLa Tet-off cells to a final volume of 10-11 ml. Make sure
Tet-On Binary Systems for Tissue-Specific and Inducible Transgene Expression
of transgenic lines have been produced akin to those found in mouse and zebrafish. Here, I describe the design and characterization of transgenic lines in Xenopus constituting the Tet-On binary transgene expression system comprising two components: (1) rtTA
The Use of Retroviral Vectors for tet-Regulated Gene Expression in Cell Populations
, the tet-system has proven to facilitate such strict control by tightly regulating gene expression, thereby assuring high expression levels in almost all organs and tissues. Yet, most of these results were obtained from the analysis of either selected cell
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