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文献和实验Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells
beaker in 1M HCl at 50-60oC for 4-16h. Cool. Wash coverslips extensively in dH2O, then ddH20. Rinse coverslips in ethanol and leave to dry between a folded sheet of whatman paper (dry as separate coverslips). Keep in a sterile tissue culture
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells
to glass. Heat coverslips in a loosely covered glass beaker in 1M HCl at 50-60oC for 4-16h. Cool. Wash coverslips extensively in dH2O, then ddH20. Rinse coverslips in ethanol and leave to dry between a folded
患者体内Ig比例构成可发生改变,如L Kronberger等将恶性乳腺疾病患者血清中的IgG1/IgG2的比例,与健康人及良性乳腺疾病患者作了对比和统计分析,发现前者血清中IgG1水平下降,而IgG2水平上升,认为可将IgG1水平下降作为诊断乳腺癌的一个指征[77];他们的其他研究发现,这一现象在多种肿瘤普遍存在,如女性生殖系统、乳腺、大肠/直肠、头颈部及前列腺等部位发生的肿瘤[78][79][80][81][82]。 二是质的方面,表现为出现许多分子结构异常的Ig分子。Gercel-Taylor
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