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文献和实验Cell Staining for Senescence-Associated beta-Galactosidase (SA-beta-Gal) Activity
Description Cell Staining for Senescence-Associated â-Galactosidase (SA-â-Gal) Activity Procedure 1. Carefully remove the growth media from the cell culture. 2. Add an equal volume of PBS and gently mix the PBS solution
Beta-Galactosidase Staining in the Skeleton
The lacZ gene, encoding for the β-galactosidase enzyme, is widely used as a reporter gene in bone biology due to the ease of visualization in situ on whole-mount or on tissue sections. In this protocol we provide detailed methods
Beta-gal staining of eukaryotic cells in vitro
(Modification of methods of Dr. Seong-Seng Tan and Promega's "Protocols and Applications Guide") Cells previously transfected with a lac Z construct can be stained for b-galactosidase activity. 1. Wash cells 2 - 3 x with PBS (2 ml
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