LEPTINPA1052

蛋白质浓缩提取和细胞裂解产品介绍

细胞裂解液,分别可用于在变性和非变性条件下的细胞裂解产物的制备.其中,非变性裂解液(C1050)含有非离子化去污剂,盐和蛋白磷酸化酶抑制剂.它可以在非变性条件下裂解细胞并释放绝大多数细胞浆和膜蛋白,因此适用于针对内源性蛋白位点的抗体的免疫沉淀.如果抗原为不可溶性(如细胞骨架,chromatin,membrane rafts 等),或者内源性蛋白的活性位点存在于蛋白折叠结构中,则可以用变性裂解液(C1052)来提取细胞裂解产物,而且,由于体外翻译的蛋白常常趋向于形成聚合物,所以应在变性条件下进行细胞裂解产物的制备

Two different point mutations in ABL gene ATP-binding domain conferring primary imatinib resistance in a chronic myeloid leukemia (CML) patient: A cas

is helpful in clinical management of imatinib resistance. We established a very sensitive (ASO) PCR to detect mutations in an imatinib-resistant CML patient. Mutations C944T and T1052C were detected which cause complete partial imatinib resistance

你还在用小白鼠做研究？Nature Methods 奇葩模式生物大盘点

, 2018. 173(7): p. 1593-1608.e20.3. Nourizonoz, A., et al., EthoLoop: automated closed-loop neuroethology in naturalistic environments. Nature Methods, 2020. 17(10): p. 1052-1059.4. Ho, C.L.A., et al., Orientation Preference Maps in Microcebus murinus