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100
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100 UL
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文献和实验E.Z.N.A. Mag-Bind Blood DNA Protocol (1-100 ul Blood)
tubes or microplates 实验步骤 1. Add 1-100 ul blood sample to a nuclease-free microcentrifuge tube. Bring the volume up to 100 ul with Elution Buffer provided with this kit. 2. Add 100 ul Buffer MSL and 10 ul Proteinase K
Sequencing off Cosmid, BAC, PAC, and Fosmid Templates with ABI Big Dye Terminators
if Thermofidelase is used but do the hold anyway and only use Thermofidelase as a last resort. Then, with 18-22mer primers use 100 cycles of the following for the 1/4 reaction: Rapid thermal ramp to 95degC 95degC for 30 sec
Natural History Museum Protocol for EST sequencing from lamb
DMSO (i. e. 7% final) and place at -70℃(no need to snap freeze). (N. b. store chloroform with some anhydrous sodium bicarbonate in the bottom of the bottle, about 1 teaspoon per 100mls.) (3) PCR the phage insert as follows: for a 40µl reaction use
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