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文献和实验E.Z.N.A. Mag-Bind Blood DNA Protocol (1-100 ul Blood)
tubes or microplates 实验步骤 1. Add 1-100 ul blood sample to a nuclease-free microcentrifuge tube. Bring the volume up to 100 ul with Elution Buffer provided with this kit. 2. Add 100 ul Buffer MSL and 10 ul Proteinase K
Shiga Toxin B-Subunit as a Tool to Study Retrograde Transport
here StxB, composed of a homopentamer of B-fragments. In a number of cell types, Shiga toxin is transported from the plasma membrane to the endoplasmic reticulum (ER), via early endosomes and the Golgi apparatus (1 –3 ), bypassing the late endocytic
A safe method of extracting DNA from Coccidioides immitis
nitrogen, lyophilized, and ground with a mortar and pestle. About 10 mg of dry ground tissue is mixed with 400 ul lysis buffer (50 mM Tris, 100 uM NaCl, 5 mM EDTA, 1 sodium dodecyl sulfate), and incubated at 80 C for 10 min., cooled to 40 C and incubated
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