NPY2R ANTIBODYNPY2R-101AP

Assisted large fragment insertion by Red/ET-recombination (ALFIRE)―an alternative and enhanced method for large fragment recombineering

media: Ap 50 μg/ml, Km 50 μg/ml. For BACs and low copy vectors, on plates and in liquid media: chloramphenicol (Cm) 20 μg/ml, Ap 50 μg/ml and Km 15 μg/ml. Tetracycline (Tc) concentrations for selection of pSC101BADγβαA[tet] was 5 and 3 μg/ml on plates

脂筏与信号途径

中跨膜分子很少，但接触抗原后可大量出现，所募集和容纳的膜型信号分子，除了受体复合结构，还包括Src家族PTK及衔接蛋白LAT等信号分子。这些微结构域是PTK介导的蛋白―蛋白相互作用的重要部位，在受体相关信号转导的的启动上发挥作用。 进一步图示了两种最为常见的免疫细胞信号转导的下游途径。其中转录因子NF―cB的激活见于TLR、NLR、TCR、BCR相关的信号转导，并参与细胞凋亡的死亡信号途径。而MAPK途径也十分常见，包括TLR、TCR、BCR、Fc2R1等引起的细胞活化，特点是有Ras

CLONING FROM BEGINNING TO END 详细的克隆操作过程【UCSF】

,sometimes it is necessary to dephosphorylate the ends of the vector so as to prevent religation before the insert can ligate to vector.If this is necessary: VECTOR DEPHOSPHORYLATION (AP/CIP): a.after digesting (it is best to do this before running on the gel and Gene