- ￥3528
- 赛默飞（Thermo Fisher）
- MSM4-953-P0
- 2025年07月14日
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- 库存：
100
- 规格：
20 UG
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文献和实验LABELING MUSCLE ACTIN WITH IATR
,small vial. Procedure (perform under reduced light,4℃ unless otherwise noted) 1.Resuspend 10 mg lyophilized actin in 2 ml buffer 1.Be careful not to make bubbles. 2.Add DTT (100 mM stock)to 5 mM. 3.Dialyze against 250 ml buffer 1 overnight
Labeling Muscle Actin with 5-iodoacetamidofluorescein
) 5.50Ti tubes,small vial. Procedure (perform under reduced light,4℃ unless otherwise noted) 1.Resuspend 10 mg lyophilized actin in 2 ml buffer 1.Be careful not to make bubbles. 2.Add DTT (100 mM stock)to 5 mM. 3.Dialyze against 250 ml buffer
. Directions for preparation of the DNaseI column are below in Appendix . Day 1 Extraction of Actin from Chicken Brain (large scale prep from tissue) 1. Homogenize tissue (fresh or frozen) in 1X Extraction Buffer without detergents at a ratio of 100
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