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文献和实验Dual- and Triple-Color Fluorospot
, and is limited to the analysis of two cytokines. Building on the principle of ELISPOT, but using detection based on fluorescence instead of substrates, the fluorospot assay was developed (10). At present, two-color fluorospot has been described
Two-Color In Situ Hybridization of Whole-Mount Mouse Embryos
RNA in situ hybridization is a powerful technique used to identify the spatial localization of a specific RNA in a tissue section or whole tissue. In this protocol, we describe a reliable method for two-color in situ hybridization
in four different osteoblastic cell lines and in isolated neonatal mouse calvarial bone cells has been examined. In the rat osteosarcoma cell line UMR-106-01, vasoactive intestinal polypeptide (VIP, 0.001-1 microM), calcitonin gene-related peptide (CGRP, 0.3-30 nM
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