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文献和实验Detection of Phosphatidylinositol-4-Phosphate 5-Kinase Activity Using Thin-Layer Chromatography
by the PI 3-kinase, generating phosphatidylinositol 3,4,5-trisphosphate (PI 3,4,5-P3 ), a second messenger of generally unknown function; however, some isoforms of protein kinase C may be targets (2 ) as well as proteins that contain pleckstrin homology (PH
In Vivo, Site-Specific, Covalent Conjugation of Quantum Dots to Proteins via Split-Intein Splicing
. Experimental procedure includes the following steps: (1) fusion of Pleckstrin-homology (PH) domains with the N-terminus half of a split intein (IN ); (2) conjugation of the C-terminal (IC ) intein-derived peptide to streptavidin-coated QDs in vitro
. xiongye2006 谢谢,我用motif scan试了下,不论是跨膜蛋白还是它的链接分子都不存在PH结构域,是不是motif scan这个网页显示得过于简单,还是我不会读PH结构域?因为很多蛋白都有PH domain,不知道其保守区是哪一段。 ylhuang0502 预测domain/motif软件的可靠性很难说,LZ还可以试一试下面两种方法: 1。用Pleckstrin homology domain 或者PH domain
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