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文献和实验了基础。 (2)用于过继性肿瘤免疫治疗:Mei-denbauer等将大量Melan-A肽特异性CTL(42.1%),静脉输注给8例难治性恶性黑色素瘤患者,结果发现外周血单核细胞中抗原特异性CTL的比率由输注的o.01%~0.07%升至输注后的2%。而且这些细胞能在活体内存活数周,具有分泌INF-7功能,并优先聚集到肿瘤局部发挥作用。 (3)用于疫苗疗效的监测:近年用抗原肽脉冲处理的扰作为疫苗免疫机体从而诱发有效的CTL反应成为研究热点。可用同源
Delivery of Tumor-Antigen-Encoding mRNA into Dendritic Cells for Vaccination
MART-1/Melan-A as a model tumor-associated antigen for the generation of a DC-based vaccine against melanoma cancer. In addition to efficient antigen loading, it is important to obtain a maximal number of potent antigen-presenting cells
Circulating Tumor Cells as Prognostic Biomarkers in Cutaneous Melanoma Patients
without the need of separating CTC from leukocytes (PBL). We selected and optimized four mRNA biomarkers (MART-1/Melan-A, MAGE-A3, PAX3, and GalNAc-T) for detection and prediction of clinical outcome in melanoma patients. Our protocol has both high sensitivity and specificity
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