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文献和实验Expression of VHH Antibody Fragments in Saccharomyces cerevisiae
The use of Saccharomyces cerevisiae as a production host for heterologous proteins has several advantages compared to the use of a bacterial host. First, S. cerevisiae has proven to be a productive host capable of expressing many heterologous
we describe a particular selection of VHH antibody fragments from phage display libraries that were constructed from llamas immunized with different complex protein samples containing the antigen of interest. We show that this can result in binders that preferentially
纳米抗体 (nanobody,Nb), 即重链单域抗体 VHH(variable domain of heavy chain of heavy-chain antibody) ,该类抗体只包含一个重链可变区 (VHH) 和 CH2,CH3 区,相比于其他抗体,轻链天然缺失。纳米抗体晶体直径 2.5nm,长 4nm,是自然存在的可以和抗原结合的最小片段。 纳米抗体类型纳米抗体和传统抗体的优劣对比 性能 传统抗体 纳米抗体 抗体
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