- ￥2576
- 赛默飞（Thermo Fisher）
- MA553981
- 2025年07月14日
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文献和实验Primary MicroRNA Processing Assay Reconstituted Using Recombinant Drosha and DGCR8
protein DiGeorge critical region 8 (DGCR8). DGCR8 has been shown to tightly bind an Fe(III) heme cofactor, which activates its pri-miRNA processing activity. Here we describe how to reconstitute pri-miRNA processing using recombinant human Drosha and DGCR8
enzyme Drosha [29]、DGCR8 (DiGeorge critical region-8)及一个双链RNA结合蛋白[30,31,32]组成。Drosha从pri-miRNA发夹结构末端切下11个核苷酸，切割后的产物在3’端有两个碱基突出，在5’端为磷酸盐基团[29]。体外试验证实，microprocessor复合物可以从发夹结构上“量出”11个核苷酸，在单链RNA与发夹结构结合处将11个核苷酸组成的片段切下，切割后的65至75个核苷酸长度的茎环结构就叫做pre-miRNA。
Protein Components of the microRNA Pathway and Human Diseases
and function, such as the ribonucleases III (RNases III) Drosha and Dicer as well as Argonaute 2 (Ago2), appear to be essential. However, the accessory proteins of the miRNA pathway, such as the DiGeorge syndrome critical region gene 8 (DGCR8) protein
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