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文献和实验Generation of Poly(ADP-ribosyl)ation Deficient Mutants of the Transcription Factor, CTCF
to produce such mutants, demonstrated by the example of CTCF, a transcription factor. To achieve this in our study, the protein domain modified by PARylation was mapped and the amino acids, which can be potentially PARylated, selected. Mutations
聚集成 CpG 岛(CCIs),50% 以上的基因启动子都含 CCIs,一般都集中在转录起始位点。甲基的存在导致了转录过程终止,从而使这些基因沉默。其机制主要与转录因子 CTCF(CCCTC binding factor)蛋白和甲基 CpG 结合蛋白(MBP)有关。CTCF 可以控制染色质三维结构的改变,促进或抑制不同位点的基因表达,但 CTCF 不能与甲基化CpG 结合,MBP 含有甲基化 CpGs 结合域,同时能与组蛋白去乙酰化酶(HDAC)结合,可重构染色质并抑制转录。 图
their findings in the January 9, 2004, issue of the journal Science. The researchers sought to pinpoint the regulatory role of a protein known as CTCF, which is believed to attach to a control region near imprinted genes. By binding to this region
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