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文献和实验细菌RNA的提取--ISOLATION OF RNA FROM BACTEROIDS
. at -80℃.ISOLATION OF RNA FROM FREE-LIVING RHIZOBIACells from 100 ml culture in early log phase GYPC were spun and resuspended in 1 ml sterile 25% sucrose. This solution was transferred to a sterile centrifuge tube and 6.5 ml M-STET, 0.5 ml lysozyme (10 mg
ISOLATION OF RNA FROM BACTEROIDS
. at -80 C. ISOLATION OF RNA FROM FREE-LIVING RHIZOBIA Cells from 100 ml culture in early log phase GYPC were spun and resuspended in 1 ml sterile 25 sucrose. This solution was transferred to a sterile centrifuge tube and 6.5 ml M-STET, 0.5 ml
ISOLATION OF RNA FROM BACTEROIDS
. Redissolve RNA in DEPC-treated H2O, ppt. with 2.5 vol ethanol, and store as ppt. at -80 C. ISOLATION OF RNA FROM FREE-LIVING RHIZOBIA Cells from 100 ml culture in early log phase GYPC were spun and resuspended in 1 ml sterile 25% sucrose
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