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文献和实验Flow Cytometry Analysis of Transcription Factors in T Lymphocytes
and convenient detection of the transcription factor BCL11B in T lymphocyte subpopulations using flow cytometry. The optimal protocol employs saponin and Tween 20 both during the fixation and permeabilization steps, and we demonstrate that it is efficient
Construction of Gene-Targeting Vectors by Recombineering
in all cells, in specific cells/tissues, or at certain developmental stages. Here we describe construction of a Bcl11b gene cko targeting vector for a multipurpose allele that can serve as a conventional knockout (ko), a cko, and a reporter allele
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