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文献和实验with purified proteins. The methods described here overcome these problems. Using a mammalian expression system, a chimeric protein comprising the extracellular domain of E-cadherin fused at its C-terminus to the Fc domain of human IgG1 (E-cadherin∶Fc
【申请上传】Current Protocols in Immunology
4.8 Construction of Human-SCID Chimeric Mice Unit 4.9 Use of the Intracellular Fluorescent Dye CFSE to Monitor Lymphocyte Migration and Proliferation Chapter 5 Immunofluorescence and Cell Sorting Introduction Unit 5.1 Overview of Flow Cytometry Unit 5.2
) 10 pmol/µl biotinylated forward PCR primer oJS2581: 5′‐biotin‐TCTAGAGAAGACAAGAACCTGACC‐3′ 10 pmol/µl reverse PCR primer oJS2582: 5′‐GGATCCGGTCTCTTAAGGCCGTGG‐3′
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