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文献和实验(ESI–TOF). Cetuximab is a potent chimeric mouse/human antibody worldwide approved for the treatment of colon and head and neck cancers. This antibody, produced by SP2/0 murine myeloma cells, is N-glycosylated both in the Fc and Fab moieties
We present here an analytical protocol for the sensitive, specific, and accurate absolute quantification of cetuximab, a human:murine chimeric monoclonal antibody, using mass spectrometry. Extraction from human serum is performed
【翻译】Development trends for monoclonal antibody cancer therapeutics
numerous ways to design mAbs that are more robust and efficacious compared with the original murine versions11. Techniques suitable for generating chimeric and humanized mAbs that contain protein sequence from both human and murine sources were developed
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