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文献和实验Which housekeeping genes for qRT-PCR control-Real-Time
housekeeping genes, like 18 S rRNA or GAPDH? thanks -smurray- I use beta-actin for our application, it seemed the most widely-used. I would encourage you to read this plug for 18srRNA : ...it makes some good points good luck
subsets, all noncovalently associated with 12 kd beta 2 microglobulin Function: restrict T cell responses to certain antigens; may mediate thymic T cell development Positive staining (normal): cortical thymocytes (70%), activated T cells
Methods 2000NOV 20:46(1-2):69-81 这里还有一些经常被使用的看家基因:beta-actin, GAPDH, cyclophilin,18sr RNA ,phosphoglyserokinase,beta-microglobulin,beta-glucronidase,hypoxanthine ribosyl transferase,transferring receptor 及另外一些。要注意看家基因可以被反应调节所影响 ,在设计定量表达研究时,保证初始对照基因的质量是必须
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