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文献和实验Preparation of Myristoylated Arf1 and Arf6 Proteins
ADP-ribosylation factor (Arf) proteins were first identified as cofactors for cholera toxin-catalyzed ADP-ribosylation of the heterotrimeric G-protein Gs. Subsequent cloning led to the discovery that Arfs were part of a group of GTP-binding
:其中 A=N-乙酰氨基葡糖,F=岩藻糖,G=半乳糖,M=甘露糖,S=唾液酸,GU=葡萄糖单位。图 4. 在 3 -MA 处理和不同渗透压下分批补料的 C44 和 C56 培养物中产生的 MAb 重链的 HPLC 聚糖谱。3 结论在这项研究中,研究了逐渐增加渗透压的影响。这项研究通过证明发现逐渐增加补料批次培养物的渗透压,可以通过在培养后期增加 qp 之前使细胞生长至高密度来使 MAb 产量增加一倍。对于表达嵌合抗体的 CHO-DUKXB 细胞系,逐渐增加分批补料培养物的渗透压浓度也使蛋白质的产量增加
Arf6 and its Role in Cytoskeletal Modulation
phospholipase D (PLD), and activating phosphatidylinositol 4-phosphate 5-kinase (2 ). However, since each Arf is broadly expressed, they must serve specific functions in cells. Our goal has been to define these functions in cells. Of these proteins, Arf1
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