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文献和实验our extensive experience with B-PER for isolating fusion proteins in which the fusion partner is either IPIII-His (an internal protein of bacteriophage T4 with a His tag at the C-terminus)or MBP (the E.coli maltose binding protein).The two cases illustrate
当然啦,不只是蛋白组学会议,还有一些RNA干扰等方面的东东. Meetings Diary RNAi 3–4 November 2003, Boston, MA, USA Website: http://www.lifesciencesinfo.com/email/3003/ Future Challenges for Target Validation 3–4 November 2003, London, UK Website: http://www.marcusevans
(bottom) clones activating VE-cadherin To verify that the selected TFZF s bound their predicted DNA substrates in vitro , we expressed the zinc-finger binding domains as C-terminal fusions with bacterial maltose-binding protein (MBP). The DNA-binding
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