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文献和实验CD74 分子 CD74 常用单克隆抗体或代号: LN2,BU- 43;(li,lg ) 主要表达细胞: B,Msub [B] 分子质量(kDa)和结构: gp41/35/33,Ⅱ类相关恒定链(li链) 功 能: 与新合成MHCⅡ类分子结合,防止MHC结合内源肽 CD74 Gamma chain antigen associated with MHC class II antigen
: 1. Prepare Whole Cell Extract. Make sure every solution DOES NOT contain DTT. 2. Add 1 or 2 μl of non-diluted monoclonal antibody (4B4 is better than 10H8 for HSF1) or 1/10 diluted polyclonal antibody to 10 μg of whole cell extract
Western Analysis of Transgenic Plants
are transferred to a solid support, which is normally nitrocellulose or polyvinylidene fluoride (PVDF [Millipore, Bedford, MA]). Immunological detection of proteins transferred to a solid support combines ease of handling with accessibility of antibodies
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