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文献和实验- - - - 1× HEPES - - - - 15mM Activin A 100ng/ml 100ng/ml 100ng/ml 100ng/ml - C687 FGF-4 - - - 500ng/ml - CR08 Wnt3a - - - 500ng/ml - C06D/C18K Noggin - - - - 100ng/ml CB89
: 1. Prepare Whole Cell Extract. Make sure every solution DOES NOT contain DTT. 2. Add 1 or 2 μl of non-diluted monoclonal antibody (4B4 is better than 10H8 for HSF1) or 1/10 diluted polyclonal antibody to 10 μg of whole cell extract
the role of Smads and ATF-2 in cardiomyocyte differentiation of P19CL6, a clonal derivative of murine P19 cells. Although P19CL6 efficiently differentiates into cardiomyocytes when treated with dimethyl sulfoxide, P19CL6noggin, a P19CL6 cell line
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