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文献和实验摘要 Trafficking of AMPA receptors (AMPARs) is regulated by specific interactions of the subunit intracellular C-terminal domains (CTDs) with other proteins, but the mechanisms involved in this process are still unclear. We have found that the GluR
Expression of NMDA Receptor Channel Subunit Proteins Using Baculovirus and Herpesvirus Vectors
(β2)/GluR6 12–14 ζ1/NMDAR1 15–17 ε1 18
: 1. Prepare Whole Cell Extract. Make sure every solution DOES NOT contain DTT. 2. Add 1 or 2 μl of non-diluted monoclonal antibody (4B4 is better than 10H8 for HSF1) or 1/10 diluted polyclonal antibody to 10 μg of whole cell extract
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