- ￥5258
- 赛默飞（Thermo Fisher）
- MA537663
- 2025年07月16日
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文献和实验Inducible raptor and rictor Knockout Mouse Embryonic Fibroblasts
of both complexes, requires genetic inhibition in addition to pharmacological inhibition. Taking advantage of the Cre/LoxP system, we generated inducible knockout mouse embryonic fibroblasts (MEFs) deficient for either the mTORC1-specific component raptor (iRapKO
: 1. Prepare Whole Cell Extract. Make sure every solution DOES NOT contain DTT. 2. Add 1 or 2 μl of non-diluted monoclonal antibody (4B4 is better than 10H8 for HSF1) or 1/10 diluted polyclonal antibody to 10 μg of whole cell extract
Inhibition of PI3K-Akt-mTOR Signaling in Glioblastoma by mTORC1/2 Inhibitors
, and siRNA against raptor, rictor, or mTOR, and evaluated the value of mTOR inhibitors for the treatment of glioblastoma.
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