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文献和实验Detection of K-ras and p53 Mutations by Mutant-Enriched PCR-RFLP
, since they fulfill many criteria that link their de novo appearance to the process of field cancerization (4 ). Oncogene mutations of the ras - and p53 -type are found in chronic smokers and patients with preneoplastic and neoplastic lesions
Interfering with Ras Signaling Using Membrane-Permeable Peptides or Drugs
During the last two decades since the first oncogene product called v-Src was identified as a constitutively activated mutant of a normal mitogenic gene (proto-oncogene) encoding a Tyr kinase called c-Src in early 1980s, it was firmly
种(如 EGFR 突变、ALK/ROS1 重排、KRAS 突变、和 BRAF 突变等),利用基因编辑技术在小鼠上引入该突变基因,使小鼠自发肺癌表型,这类模型可用于肺癌发生与转移机制的研究,以及抗肿瘤药物的筛选和评价。下面小编将主要为大家介绍两种常见的自发肺癌模型。 KRAS 突变肺癌模型 KRAS 基因是首个被确定的原癌基因,其编码的基因是一种小的 GTP 水解酶,通过在激活(GTP 结合)和失活(GDP 结合)构象之间循环来控制多个信号级联通路,与 HRAS、NRAS 同属于 Ras 超蛋白
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