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文献和实验Detection of K-ras and p53 Mutations by Mutant-Enriched PCR-RFLP
Early diagnosis of lung cancer is critical, as most cases are already inoperable at the time of diagnosis, and thus bear a grave prognosis. With increasing knowledge of the genetic aspects of lung cancer, the field has also experienced
Detection and Characterization of Oncogene Mutations in Preneoplastic and Early Neoplastic Lesions
oncogene has the potential to serve as a useful biomarker in the early diagnosis and risk assessment of cancers with oncogenic Ras signaling. This chapter describes a highly sensitive method for detecting mutant K-RAS , enriched PCR, and its application
Methods for Isolation and Genetic Analysis of Circulating Tumor DNA in Patient Plasma
that 7 of 14 colorectal cancer patients (50%) had a codon 12 K-Ras mutation within their primary tumor, and identical mutations were found in the plasma DNA of 6 of those 7 patients (86%). Mutant DNA was not found in the plasma specimens of 7 patients
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