- ￥2827
- 赛默飞（Thermo Fisher）
- MA517540
- 2025年07月10日
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文献和实验Western Blotting with Horseradish Pe
. 3.Centrifuge the homogenate (16,000 x g, 15 C) for 5 minutes to pellet insoluble material, then discard pellet. 4.Dilute an aliquot of the tissue lysate sample at least 10-fold for the BCA (Pierce) protein concentration assay. POLYACRYLAMIDE GEL
according to genotype. Eur.J.Immunol. 5(2):112-117, 1975. Yokoyama , W.M. Natural killer cells and the NK gene complex. In: Cell surface and messenger molecules of the immune system , edited by Weir, D.M., Herzenberg, L.A., and Blackwell, C.Cambridge, MA
media (= RPMI 1640 + 10% FBS) PBS + 2% EDTA Triton X-100 solution (10% in dH2 O) Effector cell preparation: The mouse NK-cell line KY1 is used as an effector cell. During recovery (e.g. after freezing), these cells require 1000 U
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