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文献和实验to avidin magnetic beads (Dynal™). The final elutes, following the binding of the biotin linked RNAs with the avidin magnetic beads and washing, were then DNAse, RNAse H or RNAse A treated, RT (BioRad™ iScript) reacted and PCR amplified. References 1.Kim
, dry 5. block half of the plate (with platelets) by PBS-BSA 1% and coat another half by glycine (x1) - BSA for 1 hour at 37ѓC 6. wash 3 times in PBS-tween 20, dry 7. incubated at 37ѓc for 1 hour with a 1:1000 dilution of rat IgG2a anti-mouse kappa
我准备做凋亡过程中CASPASE 3的变化,用WESTERN检测。我准备买SANTA CRUZ的抗体。有两种,一种是单抗,另一种是多抗。具体如下: 1.caspase-3 (E-8): sc-7272 SOURCE Caspase-3 (E-8) is a mouse monoclonal IgG2a antibody corresponding to amino acids 1-277 representing the full length precursor form
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