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文献和实验in detail here. The first protocol is essentially a dual-pulldown assay employing Flag-tagged DNMT3A or antibody of choice for an endogenous protein and 5' biotin linked antisense RNA (Figure 1a), while the second protocol is a triple pulldown assay which essentially expands
PRINS for the Detection of Gene Deletions in Cancer
of the RB1 and p53 tumor suppressor genes, located in 13q14 and 17p13, respectively, frequently occur in leukemias; this has been confirmed by molecular methods such as fluorescence in situ hybridization (FISH). Our group modified the primed in situ labeling
may be biotinylated where a streptavidin-peroxidase conjugate binds to the biotin on the secondary antibody, greatly increasing the sensitivity for protein detection. E: Visualization For the chemiluminescent reaction, incubate with equal parts
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