- ￥3017
- 赛默飞（Thermo Fisher）
- 14-0431-85
- 2025年07月13日
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文献和实验In Situ Hybridization Using Digoxigenin Labeled Probes
on two 5ml pipettes in a tray lined with moist paper towels. Apply 100µl of a 1:500 dilution of Anti Digoxigenin-POD in 1X PBS, 5mMEDTA. Distribute the solution by placing a 22 x 40 mm coverslip on the slide. 5) Cover the tray with plastic wrap
分离培养大鼠或小鼠小脑神经元 Dissociated Cultures of Cerebellar Neurons
well: 5 X 105 live cells 0.5 ml 35 mm: 2.5 X 106 1.5 ml 60mm: 5 X 106 3 ml 100mm: 1.5 X 107 9 ml note, 24w: shake plates vigorously left-right, top-bottom, before placing into incubator, to evenly distribute cells donÕt need to change media after seed
Calcium Phosphate Transfection of Neurons in Primary Culture
: 24 well: 2-4ug (eg., for immunostaining) 60mm: 2-5ug (eg., for RNAase protection) recipe: eg., for 300ul precipitate (scale up or down accordingly): in a 15ml polystyrene pop-cap tube
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