- ￥4925
- 赛默飞（Thermo Fisher）
- MA182873
- 2025年07月14日
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文献和实验,and the cell pellets were frozen and stored at –80ºC. Lysis Lysis was accomplished with B-PER reagent supplemented with 1/50 vol 10-mg/ml chicken egg-white lysozyme (stored at –80ºC in aliquots),1/125 vol 5-mg/ml DNaseI (in 20 mM sodium acetate,pH adjusted
minutes at 4°C. Discard the supernatant. 3. Dissolve the pellet in 1 ml of lysate buffer. Bring to 1 mM PMSF by adding 100 mM PMSF stock (17.42 mg/ml in methanol). This solution may be kept for 1 week at -20°C. 4. Add 50 µl of a 10 mg/ml lysozyme
by centrifugation, and resuspend the cell pellet in Solution I (without lysozyme). Use 25 ml Solution I per liter culture. 3)Add lysozyme to 2.5mg/ml, and mix by inversion. 4)Add Solution II (50 ml per liter culture) and mix well by inversion. Leave on ice for 10
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