- ￥5014
- 赛默飞（Thermo Fisher）
- MA125057
- 2025年07月14日
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文献和实验Immunofluorescent Localization of Tubulin
antibody to tubulin) FITC-labeled goat anti-rabbit (or secondary antibody to match the primary) Fluorescence Mounting Media Fluorescent Microscope equipped with 490 nm excitation filter and 515 nmbarrier filter Kodachrome film
contamination in the final tubulin stock. 6. Resuspend pellet in 1-2 ml of ice cold IB (the exact volume will depend on the concentration and polymerizability of the tubulin in the column fractions). Transfer the chunky pellet in IB to an ice-cold
Large Scale Tubulin Preparation
Tubulin is purified from bovine/porcine brain by two cycles of polymerization/depolymerization followed by removal of copurifying proteins on a phosphocellulose (PC) column. The procedure described here is for a large scale prep (10 cow brains
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