- ￥4219
- 赛默飞（Thermo Fisher）
- MA119401
- 2025年07月15日
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MOUSE MONCLONAL ANTIBODY-
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文献和实验make an Antibody against a Peptides
to create an immunogen. One-step chemical reactions are easy with Pierce Sulfo-SMCC Pre-Activated Carrier Proteins (Figure 1). If your hapten does not have a sulfhydryl group, you can use EDC chemistry (Figure 2) to couple amines (lysine) or carboxylic acids
, bleeding procedure, obtaining serum, precipitation of IgG. ・ GST-fusion protein injection into rabbits (IVAN J. DELGADO ORLIC) ・ How Do I make an Antibody against a Peptides (Pierce) ・ A fast and inexpensive method
were resolved in 4 to 20% SDS-polyacrylamide gels and electrophoretically transferred to nitrocellulose filters from Schleicher & Schuell (Keene, NH) for 2 h at 140 mA. The filters were blocked with blocking buffer (TTBS; 150 mM NaCl, 50 mM Tris, 0.2% Tween 20
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