- ￥5245
- 赛默飞（Thermo Fisher）
- MA119187
- 2025年07月08日
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MOUSE MONOCLONAL ANTIBODY-
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文献和实验, Buckinghamshire, UK). 2.3. Immunoblotting 1. Transfer buffer (10×): 250 mM Tris-Base and 1.92 M glycine. Store at RT. 2. Polyvinylidene difluoride (PVDF) membrane from Millipore(Bedford, MA) and gel blot paper from ISCBioExpress(Kaysville and UT
make an Antibody against a Peptides
to create an immunogen. One-step chemical reactions are easy with Pierce Sulfo-SMCC Pre-Activated Carrier Proteins (Figure 1). If your hapten does not have a sulfhydryl group, you can use EDC chemistry (Figure 2) to couple amines (lysine) or carboxylic acids
, bleeding procedure, obtaining serum, precipitation of IgG. ・ GST-fusion protein injection into rabbits (IVAN J. DELGADO ORLIC) ・ How Do I make an Antibody against a Peptides (Pierce) ・ A fast and inexpensive method
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