- ￥5131
- 赛默飞（Thermo Fisher）
- MA119579
- 2025年07月09日
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文献和实验Which housekeeping genes for qRT-PCR control-Real-Time
housekeeping genes, like 18 S rRNA or GAPDH? thanks -smurray- I use beta-actin for our application, it seemed the most widely-used. I would encourage you to read this plug for 18srRNA : ...it makes some good points good luck
Protocol for intracytoplasmic staining of cytokines for FACS analysis
; Pharmingen 18195A) for FL2 anti-IL10 (JES5.16E3-PE; Pharmingen 18435A) for FL1 anti-TGF-beta (rabbit anti pan TGF-beta; R&D Systems AB-100-NA home made FITC conjugate) for FL2 8) Wash extensively in saponin buffer followed by PBS + 0.1% azide
Co-staining with Thioflavin S & Carboxyfluorescein
Molecular Probes,1mM w/DMSO) for 30 minutes 7.) Wash with F12+ twice 8.) View immediately under the fluorescent microscope using FITC filter. 9.) Fix with 2% formalin for 20 to 30 minutes 10.) Coverslip with water-soluble mounting media
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